Compare BoVir^®
Simply Superior Results

BoVir® is a state-of-the-art assay with none of the overpooling commonly used in other tests that can generate unreliable outcomes. In fact, extensive European tests prove BoVir® delivers superior results in all 68 referenced strains, including atypical strains, such as the HOBI and H138 strains, two strains most recently identified in the U.S.

BoVir® is real-time Reverse Transcriptase Polymerase Chain Reaction (real time RT-PCR), but with a unique, proprietary lysis buffer and reagents to improve the accuracy and turnaround time of test results.

Compare BoVir® and you’ll see why there’s really no comparison.

BoVir® - real time Reverse Transcriptase Polymerase Chain Reaction with Lysis Buffer & Reagents

STRENGTHS

Easy to use - simple process providing results in under 4.5 hours, with one lab tech at 1,000 samples per day
Fast ear notch preparation: homogenate pipetted straight from vial to plate with no additional RNA extraction necessary
Proprietary lysis buffer and reagents improve on real time RT-PCR to eliminate incorrect handling that can lead to false positives and negatives
Two-component, one step real time RT-PCR master mix
Higher analytical sensitivity to previously published methods by a factor of 50-500
Enhanced sensitivity allows pooling for European governmental-approved protocols
Detects 10-100 viral particles in 1ml of whole blood
95 percent detection in C_T values of 23-28
Differentiation of persistent infection vs. transient infection in blood samples

ELISA – Enzyme Linked Immunosorbent Assay
STRENGTHS	WEAKNESSES
Longtime, broad use for a wide variety of assays, both human and veterinary Common throughout many labs	Time- and labor-intensive; reduces high throughput Overnight protocol Reliability depends on complex, tedious lab techniques that are prone to human error Antibodies from vaccines and colostrums will interfere with test results creating false negatives No pooling Higher rate of false positive results Lacks sensitivity to detect acute/transient infection in blood samples Retesting is labor- and investment-intensive; unable to perform further analysis with the same ear notch sample

RT-PCR – Reverse Transcriptase Polymerase Chain Reaction.
STRENGTHS	WEAKNESSES
Proven technology used in research to detect specific DNA strands	Requires highly skilled lab techs and meticulous, uncontaminated lab environment, which impedes fast turnaround and adds costs Not designed for high throughput lab operations Lacks internal controls Questionable results with samples from newborn calves up to first 120 days Difficult to analyze results Sample pools often used to reduce the cost of testing every cow, producing inaccurate results Does not detect all strains in different genotypes

(real time RT-PCR) – real time Reverse Transcriptase Polymerase Chain Reaction.
STRENGTHS	WEAKNESSES
Extremely sensitive Greater level of specificity with real-time detection	False positives and false negatives caused by incorrect handling of reagents and specimen preparation Sample pools often used to reduce the cost of testing every cow, producing inaccurate results Lacks internal controls Questionable results with samples from newborn calves up to first 120 days Does not detect all strains in different genotypes Lacks sensitivity to detect acute/transient infection in blood samples No differentiation of transient vs. persistent infection in blood samples

Compare BoVir® Simply Superior Results

Compare BoVir^®
Simply Superior Results